Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Extracellular Vesicle Marker Changes Associated With Disease Activity in Relapsing-Remitting Multiple Sclerosis

doi: 10.1212/NXI.0000000000200404

Figure Lengend Snippet: EVs were isolated by size-exclusion chromatography (SEC, SmartSEC, System Biosciences). (A) Analysis of the classical EV marker Alix by Western blotting after isolation. Equal amounts of EVs and plasma samples (10 µg) were used. A weak level of albumin was detected in the EV fraction. (B) Freshly isolated EVs were subjected to cryo-electron microscopy (cryo-EM). Cryo-electron microscopy observations showed EVs with typical lipid bilayer membrane. EV = extracellular vesicle.

Article Snippet: Thereafter, membranes were incubated with a primary antibody against Alix (1:500, NBP1-90201, Novus Biologicals, Centennial, CO) in a rolling shaker overnight at 4°C.

Techniques: Isolation, Size-exclusion Chromatography, Marker, Western Blot, Clinical Proteomics, Cryo-Electron Microscopy, Cryo-EM Sample Prep, Membrane

Journal: Future microbiology

Article Title: Liquid biopsy in the diagnosis of HPV DNA in breast lesions.

doi: 10.2217/fmb-2017-0145

Figure Lengend Snippet: Figure 1. Serum-derived extracellular vesicles isolation and characterization. (A) Serum-derived EVs were isolated by differential ultracentrifugation and DNase-I digested, (B) Western Blot and NanoSight analysis of 8 Alix positive serum-derived EVs (see Table 2) and representative NanoSight plot (n = 3 replicates) of sample 51 (see Table 2). EV: Extracellular vesicle.

Article Snippet: The effective presence of EVs was assessed by Western Blot for Alix expression (Mouse antiALIX antibody, clone 3A9, MCA2493, Bio-Rad, Milan Italy) and EVs were counted by NanoSight NS500 (IRST, Meldola, Italy) (Figure 1B).

Techniques: Derivative Assay, Isolation, Western Blot